All entries for Thursday 05 July 2007

July 05, 2007

First two weeks…

After my rather upbeat first entry i can’t say i am as entusiastic now! I am looking at the distribution of methanogens in the guts of higher temites. presently i am working with two samples of DNA extracted frome the guts of termites; sample 001 and sample 133. At first i had to learn the biological tecniques needed to amplify the sections of DNA i was to look at.

The Polymerase Chain Reaction (PCR)
I have simplified the events of PCR so everyone can get a faint idea of what i’m doing below:
1. Heating the DNA to seperate the two strands.
2. Designed, archael specific, primers bind to both strands of the DNA.
3. Taq polymerase extends the primers to make copies of the archaeal specific DNA fragments.

I have found PCR relativley simple. As my supervisor says it is like cooking, as long as you follow the recipe it tends to work, however there has been occasions where i must have added a few ingredients of my own as it has not always been successful.

Gel Electrophoresis
This technique seperates the PCR products by size creating bands of DNA that correspond to a marker that is always used in the first well.

Gel Electrophoresis Rig

Photo of the stained DNA gel observed in UV light.

At fist i was just carrying out simple PCR’s that were guaranteed to work, such as primers used on bacterial DNA. As these seemed to work fine i progressed to archaea DNA using non specific primers and the termite gut samples, some of these did not work as well and after much persiverance i acheived the results i wanted. I have now progressed onto termited group 1 specific archaeal primers – the particular group i am interested in and will let everyone know how it goes!


July 2007

Mo Tu We Th Fr Sa Su
Jun |  Today  | Aug
                  1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31               

Search this blog

Tags

Galleries

Most recent comments

  • Which stretch of the Grand Union Canal did you cover? I'm aiming to walk the whole thing by the end … by on this entry
  • I would certainly like to do the final report and the poster presentation. (Nuffield requires me to … by on this entry
  • Yes that's great, we still want you around. Actually this really helps us, because we're planning on… by on this entry
  • >should anyone like to pay me (ESRF, some private body/ charity) >for my new hobby feel free to post… by on this entry
  • Hmmm, slightly confused, cant seem to see my week 10 entry on my blog. ?. How do you choose what cat… by on this entry

Blog archive

Loading…
Not signed in
Sign in

Powered by BlogBuilder
© MMXXI