All 1 entries tagged PhD Synthetic Clock

Synthetic Clock PhD plan

Writing about web page http://blogs.warwick.ac.uk/ulrichjanus/entry/todo_1/

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Results of the meeting with Markus and Isabelle last Friday (11.Nov). Email to get the E.Coli strain used by Atkinson 2003 is under way..

Motivating or guiding Question

• How accurately can synthetic systems be described by mathematical models?
• How well can specific parameters of the in vivo system be changed?

Main Goals:

• Review the theoretical model (kinetics and structure) to make it more realistic.
• Identify relevant parameters to move system into the region of sustained oscillations.
• Achieve this parameter shift by mutagenesis of transcription factors by error prone PCR.
• Compare responses of theoretical and in vivo system.

Postulated Results:

• Model predictions can be improved by using more realistic kinetic description of the regulation events.
• Mutagenesis by PCR allows are more precise manipulation of model parameters than the method used previously.

Schedule of the Experimental Work:

• Check if the strains are still rythmic, i.e. if the previous work can be reproduced (~ 1mth.)
• Intoduce a luciferase reporter gene to measure clock function via luminescence assays (~ 1mth.)
• Test, if cells can be grown on agar plates rather than in liquid cultures
• mutagenise the sequence of relevant transcription factor to change chosen model parameters by erroneous PCR (~ 2mth)
• Identify mutated colonies by changed clock behaviour caused by changes in the affinites of the transcription factor (~ 4mths)
  These are 8mths, make it 12mths.

about the advisor(s):
Postponed…

: 14 Nov 2005 14:53 |  Tags: PhD Proposal PhD Synthetic Clock |  Comments (0) |  Close comments |  Report a problem