June 26, 2006

Synthetic Biology talks II

Follow-up to Synthetic Biology from Schreibfaul in Warwick

Title: "Programming bacteria: wiring synthetic sensors and circuits to heterologous outputs"

talk by Christopher Voigt

Three systems in E.Coli:

  1. Light Sensor Nature 2005
  2. Heat Sensor
  3. Logic Gate. in progress. previous Leiber Science2002

Programming Bacteria:

  • Competition 6 month time limit. iGEM Genetic Engineering Competition
  • a national student competition

One: Light Sensor:

  • Bacterial Photography. Change gene expression on light.
  • on known photoreceptors in E.coli. just in other organisms.
  • Phytochromes in Cyanobacteria, Arabidopsis.
  • kinase that is activated on light induction
  • compine e.coli osmotic shock sensor with cph1, ouput lacZ

Two: Bacterial Thermometer:

  • Heat shock and Cold shock repsonse promoters
  • Cold: Hypb, ansb, cspa_x promoter
  • Heat: htpG

Synthetic Logic (AND gate):

  • Examples of other gates in ecoli, yeast and mammal cells

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Ulrich Janus

June 2006

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