*Johns-Gems*

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Essential tips & tricks from John Asburner

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## June 03, 2010

### SPM2 Gem 11: Un–normalization

This script of John's will find the corresponding co-ordinate in un-normalised image: get_orig_coord2.m

### SPM2 Gem 10: Creating a mask from a XYZ list

This is a "Jan's Gem" from Jan Gläscher...

Subject: Re: [SPM] creating a mask from voxel coordinates From: Jan Gläscher <glaescher@UKE.UNI-HAMBURG.DE> Date: Tue, 15 Mar 2005 12:24:27 +0100 To: SPM@JISCMAIL.AC.UK Dear Susana, you can try the following recipe. It might not be the easiest way, but it should work. Suppose your n MNI coordinates are saved in a 3xn matrix called mni. (This is essential (not the name, but the format of 3xn), otherwise the steps below won't work). 1. Create an spm_vol handle to the image, that you determined the coordinates from Vin = spm_vol(spm_get(1,'*.img','Select image')); 2. Read the information in the image. (The data are not needed, this is done purely for the purpose of setting up a matrix of voxel coordinates. [Y,XYZ] = spm_read_vols(Vin); 3. Setup a matrix of zeros in the dimensions of the input image mask = zeros(Vin.dim(1:3)); 4. Now loop over all voxels in your mni variable and set the corresponding location in the mask matrix to 1: for v = 1:size(mni,2) mask(find(XYZ(1,:)==mni(1,v)&XYZ(2,:)==mni(2,v)&XYZ(3,:)==mni(3,v))) = 1; end 5. Setup an spm_vol output file handle and change the filename Vout = Vin; Vout.fname = '/path/to/the/directory/mask.img'; 6. Finally, write the new mask to the output file. spm_write_vol(Vout,mask); Cheers, Jan

### SPM2 Gem 9: Masking w/ ImCalc

This is an email from Rik Henson which references John. It addresses the fact that ImCalc defaults to writing out images in 2-byte signed integer format, which is can be problematic if you're masking statistic images (which should written with floating point precision).

Subject: Masking with ImCalc From: Rik Henson <r.henson@UCL.AC.UK> Date: Fri, 14 May 2004 08:24:25 +0100 (03:24 EDT) To: SPM@JISCMAIL.AC.UK Several people have asked how to mask SPMs across different analyses. This used to be possible using ImCalc in SPM99 (see Mailbase archive). In SPM2 however, when ImCalc is called from the GUI, it adopts various defaults, which include writing images as "uint16" (rather than "float", which is the normal datatype for T/F imgs). Thanks to John for pointing this out. It is for this reason that the procedure of overwriting spmT/F*imgs using ImCalc does not work from SPM2's GUI. To override this default however, you can simply call ImCalc from the command line instead, setting the datatype to spm_type 'float': spm_imcalc_ui([],[],'i1.*(i2>3.09)',{[],[],'float',0}) The empty 1st and 2nd arguments mean that SPM will prompt you to select the input images and the output filename, as usual. You will of course need to change the third argument to your particular ImCalc equation for evaluation. The 4th argument is a cell array of defaults, which includes the 'float' enforcement. For more info, type "help spm_imcalc_ui". Rik

See also SPM2 Gem 8, and SPM99 Gems 3, 16, and 21.

### SPM2 Gem 8: ImCalc Script

SPM99 Gem 21 is the same (with minor changes).

Subject: Re: a script to use ImaCal From: John Ashburner <john@FIL.ION.UCL.AC.UK> Date: Thu, 16 Oct 2003 11:24:17 +0000 (07:24 EDT) To: SPM@JISCMAIL.AC.UK > Brain image for each subject to mask out CSF signal was generated by > using MPR_seg1.img (i1) and MPR_seg2.img (i2) with (i1+i2)>0.5 in > ImaCal.(called brainmpr.img for each subject) > > Then I have more than two-hundred maps, which need to mask out > CSF. I think I can use ImaCal again with selecting brainmpr.img (i1) > and FAmap.img (i1), and then calculating (i1.*i2) to generate a new > image named as bFAmap.img. Unfortunately, if I use the ImaCal, it > take so long time to finish all subjects. Could anyone have a script > to generate a multiplication imaging with choosing a brain > image(brainmpr.img, i1) and a map image (FAmap.img, i2) and writing > an output image (bFAmap.img) from i1.*i2? You can do this with a script in Matlab. Something along the lines of the following should do it: P1=spm_get(Inf,'*.IMAGE','Select i1'); P2=spm_get(size(P1,1),'*.IMAGE','Select i2'); for i=1:size(P1,1), P = strvcat(P1(i,:),P2(i,:))); Q = ['brainmpr_' num2str(i) '.img']; f = '(i1+i2)>0.5'; flags = {[],[],[],[]}; Q = spm_imcalc_ui(P,Q,f,flags); end; Note that I have not tested the above script. I'm sure you can fix it if it doesn't work. Best regards, -John

### SPM2 Gem 7: Extracting realignment parameters

The referenced script is here save_parameters.m

Subject: Re: [SPM] realignment parameter From: John Ashburner <john@FIL.ION.UCL.AC.UK> Date: Fri, 23 Apr 2004 11:25:27 +0000 (07:25 EDT) To: SPM@JISCMAIL.AC.UK > I have switched from spm99 to spm2 and am learning new things. > When I realign images interactively with GUI interface, > the rp*.txt file is created. However, the parameter file > is not created - or, I cannot find it -, when I realign > with a batch script. > I am sure I left out something, but I don't know it. > Any suggestion will be very appreciated. If you call spm_realign with output (left-hand) arguments, then no rp_*.txt file is saved. You could create a save_parameters.m file, containing: %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% function save_parameters(V) fname = [spm_str_manip(prepend(V(1).fname,'rp_'),'s') '.txt']; n = length(V); Q = zeros(n,6); for j=1:n, qq = spm_imatrix(V(j).mat/V(1).mat); Q(j,:) = qq(1:6); end; save(fname,'Q','-ascii'); return; %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% and derive the values from your realigned images: P = spm_get(Inf,'*.IMAGE','Select realigned images'); save_parameters(spm_vol(P)) Best regards, -John

### SPM2 Gem 6: Contrast SE Computation

An email from Will Penny on how a contrast's standard error is computed.

Subject: Re: Beta SE in SPM2? From: Will Penny <wpenny@FIL.ION.UCL.AC.UK> Date: Fri, 27 Feb 2004 11:26:35 +0000 To: SPM@JISCMAIL.AC.UK Tobias, > How do I extract beta SE in SPM2? As far as I can see I can only get > y, Y, beta, and Bcov...have I missed something? If you're referring to the Standard Error or the Standard deviation of the parameter estimates then this isn't stored explicitly. Its computed on the fly when one makes an inference about a contrast. See the following lines (153-158) in spm_contrasts: case 'T' %-Compute SPM{t} image %--------------------------------------------------------------- cB = spm_get_data(xCon(ic).Vcon,XYZ); l = spm_get_data(VHp,XYZ); VcB = xCon(ic).c'*SPM.xX.Bcov*xCon(ic).c; Z = cB./sqrt(l*VcB); So, if you did a contrast xCon(ic).c'=[1 0 0 0] to test the null hypothesis that the first parameter is zero then cB will be equal to beta1 and sqrt(l*VcB) will be equal to std(beta1) - the standard deviation of the first parameter estimate. Note that l is the residual sum of squares at that voxel which is stored in the image ResMS.img and accessed via the file handle VHp. In this way SPM does'nt have to store Standard Deviations/covariances for every voxel and beta - so saving disk space. But its not that handy for what you want ! Hope this helps, Will.

### SPM2 Gem 5: Normalizing [x y z]

An off-list email reports how to find the point in an normalized image that corresponds to a given un-normalized location. See also Un-normalizing a point.

From: John Ashburner <john@fil.ion.ucl.ac.uk> To: Christophe Phillips <c.phillips@ulg.ac.be> Subject: Re: Coordinates transform Date: Wed, 4 Feb 2004 17:26:21 +0000 Hi Christophe, > I've the coordinates [x y z] of a point (actually the location of a > fitted current dipole) in an image 'img1'. This image was normalised > into 'w_img1'. I'd like to have the dipoles coordinates [x y z]_w in > the normalised image... > > Do you have a routine that can do that easily from the 'img1_sn.mat' > file ? The deformations in the sn.mat files map from points in normalised space, to points in un-normalised space. You therefore need to invert them, which is a bit messy. You would use the Deformations toolbox for this (from the Toolbox pulldown). First write out the deformation field from the sn.mat. Then call the Deformations toolbox again, and choose the deformations inversion option. This will be an iy*.img file with three volumes. This little script can then be used. c = [x y z]'; % co-ordinates in mm (as shown by Display) P = spm_get(1,'iy*.img','Select inverted warp'); P = [repmat(P,3,1) [',1';',2';',3']]; V = spm_vol(P); vx = inv(V(1).mat)*[c ; 1]; % The voxel in the deformation to sample w_coord = [... spm_sample_vol(V(1),vx(1),vx(2),vx(3),1) spm_sample_vol(V(2),vx(1),vx(2),vx(3),1) spm_sample_vol(V(3),vx(1),vx(2),vx(3),1)] I haven't tested it, so there may be things to fix. All the best, -John

### SPM2 Gem 4: –log10 P–values from T images

P-value images are difficult to visualize since "important" values are small and clumped near zero. A -log10 transformation makes for much better visualization while still having interpretability (e.g. a value of 3 cooresponds to P=0.001).

This function, `T2nltP`, will create -log10 P-value image based on either a contrast number (which must be a T contrast) or a T statistic image and the degrees of freedom.

This version for SPM2 was created Michael Moffitt, based on the SPM99 function of the same name for SPM99. Note that Michael's version can easily be changed to create P-values instead of -log10 P-values.

function T2nltP(a1,a2) % Write image of P-values (spm_nltP_?) for a T image % % FORMAT T2nltP % SPM will ask you which spmT_? file you want to convert to spm_nltP_? % % FORMAT T2nltP(Timg,df) % Timg Filename of T image % df Degrees of freedom % % % As per SPM convention, T images are zero masked, and so zeros will have % P-value NaN. % % @(#)T2nltP.m 1.2 T. Nichols 03/07/15 % Modified 04/01/20 by MAM - for SPM2 compatibility if nargin==0 % Ask user for SPM.mat file and specific contrast [SPM,xSPM]=spm_getSPM; % If a 'T' contrast, get degrees of freedom (df) and fname of spmT_? if xSPM.STAT ~= 'T', error('Not a T contrast'); end df=xSPM.df(2); Tnm=xSPM.Vspm.fname; elseif nargin==2 Tnm = a1; df = a2; end Tvol = spm_vol(Tnm); Pvol = Tvol; Pvol.dim(4) = spm_type('float'); Pvol.fname = strrep(Tvol.fname,'spmT','spm_nltP'); if strcmp(Pvol.fname,Tvol.fname) Pvol.fname = fullfile(spm_str_manip(Tvol.fname,'H'), ... ['nltP' spm_str_manip(Tvol.fname,'t')]); end Pvol = spm_create_vol(Pvol); for i=1:Pvol.dim(3), img = spm_slice_vol(Tvol,spm_matrix([0 0 i]),Tvol.dim(1:2),0); img(img==0) = NaN; tmp = find(isfinite(img)); if ~isempty(tmp) % Create map of P values %img(tmp) = (max(eps,1-spm_Tcdf(img(tmp),df))); % Create map of -log10(P values) img(tmp) = -log10(max(eps,1-spm_Tcdf(img(tmp),df))); end Pvol = spm_write_plane(Pvol,img,i); end; spm_close_vol(Pvol);

### SPM2 Gem 3: Finding p–value and statistics for all voxels

(Note: The program in this email was corrected; I have added the corrections to this message. Also, an additional typo was discovered in Sep 2003 and was fixed.)

Subject: Re: tabulate all voxels of a volume or cluster From: John Ashburner <john@FIL.ION.UCL.AC.UK> Date: Thu, 27 Mar 2003 14:26:56 +0000 (09:26 EST) To: SPM@JISCMAIL.AC.UK > Is there a possibility to tabulate the p-values and statistics for all > voxels for the entire volume. (not only the peaks) ? > Or which data file should I readout to get the desired > values? Copy and paste the following into Matlab, and select the appropriate spmT or spmF images. The first 3 columns are MNI coordinates. The fourth is the statistic. I haven't included any p-values, but this would be a relatively simple operation, provifing you can figure out degres of freedom etc. Use spm_Tcdf.m or spm_Fcdf.m to do this. P=spm_get(1,'*.img','Select statistic image'); V=spm_vol(P); [x,y,z] = ndgrid(1:V.dim(1),1:V.dim(2),0); for i=1:V.dim(3), z = z + 1; tmp = spm_sample_vol(V,x,y,z,0); msk = find(tmp~=0 & finite(tmp)); if ~isempty(msk), tmp = tmp(msk); xyz1=[x(msk)'; y(msk)'; z(msk)'; ones(1,length(msk))]; xyzt=V.mat(1:3,:)*xyz1; for j=1:length(tmp), fprintf('%.4g %.4g %.4g\t%g\n',xyzt(1,j),xyzt(2,j),xyzt(3,j),tmp(j)); end; end; end; Best regards, -John

### SPM2 Gem 2: Making your own MINC SPM2 templates

Subject: Re: FDP template --- SPM99 version

From: John Ashburner <john@FIL.ION.UCL.AC.UK>

Date: Thu, 27 Mar 2003 16:53:03 +0000 (11:53 EST)

To: SPM@JISCMAIL.AC.UK

> Those who use the FDG template should know that is was created for SPM99,

> and is thus in the standard orientation for that program, which is the

> opposite from that used in SPM2b.

>

> I believe that John Ashburner has agreed to convert to MINC format for use

> in SPM2b.

Converting from SPM99 to SPM2b templates can be pretty easy. In this case, the

command was (all one line):

rawtominc -transverse -short -signed -range 0 32767 -real_range 0 32767

-orange 0 32767 -xstep 2 -ystep 2 -zstep 2 -xstart -90 -ystart -126

-zstart -72 -xdircos 1 0 0 -ydircos 0 1 0 -zdircos 0 0 1 -pet FDG.mnc

91 109 91 < template_FDG_PET_OSEM_seg.img

The converted template can be downloaded from:

ftp://ftp.fil.ion.ucl.ac.uk/spm/spm2b_updates/templates/

rawtominc can be obtained from:

ftp://ftp.bic.mni.mcgill.ca/pub/minc/

Note that the simple conversion only applies to SPM99 templates in

Analyze format. If you do the same with SPM2b generated templates,

then you will need to do a left-right flip to them first.

Best regards,

-John