All 9 entries tagged Stuff No-One Else Will Ever Be Bored Enough To Read

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October 26, 2006


...were I not a stupid humanoid, I could be such a hawt car.

Mia Car... geddit?

Ka-chow! ;)

M xxx

April 18, 2006

IQ Tests

Small Chocolate Teapot

…Are teh suck.

If I'm so smart why am I headed straight for the jaws of disaster? Eh? Stoopid test…

She says while singing loudly, sucking on a candy-cane and msn-ing carefully as she waits for her nailvarnish to dry.

Oh and revision. she does a bit of that in the gaps inbetween.

Bring it on.

M xxx

April 17, 2006

About as useful as a…

Follow-up to Every time you miss a post… from Something Random

Chocolate TeapotIt's happening again. That urge to post and post and post again. Not only out of boredom and procrastination but through the sheer compulsion to underline every day of the week. I can't help it. I've made it over a week now with every day filled and I think I'm addicted. Again. Goddamn.

So in anticipation of the future piss-poor posts brought about by this sad condition I have developed this rather amazing visual warning cue. Its appearance indicates that a particular post is contributing about as much to the furthering of mankind as a chocolate teapot, and need not be read unless you seriously have nothing to do with your life or happen to be recovering from a full frontal lobotomy. Thus hopefully aiding blog browsing.

And it still keeps everything on a tea theme. (Well, excluding the dead bunnies i guess)

That is all. As revision demands my sorry ass back in the kitchen.

M xxx

lines... ooooh the linessss...

April 09, 2006

Seeking a second opinion

Is it just me…

… or does the guy below look like he's just finished slaughtering someone\thing before going merrily on his way and frying some potatoes to celebrate. Such a creepy smile, he's got a homicidal glint in his eyes I swear. Oh the blood, so much blood…

Bloody crisps

Mmmmmmmmm soylent potato…

M xxx

December 13, 2005

Strangely comforting

Chocolate Teapot

Which Fantasy/SciFi Character Are You?

Putting your appointed path ahead of any inner conflicts, you make your own rules for the benefit of all.

If my life or death I can protect you, I will.

Awwww, yay me, I have a beard, but unfortunately according to the stats I am awfully common…

M xxx

October 29, 2005

Developmental Biology Tutorial No. 2 – The Making of a Vertebrate Limb.

"How is the vertebrate limb patterned?"

If you do not wish to read about vertebrate limb development it is advised you scroll straight down to the kittens strategically placed at the bottom of the page to help you overcome the trauma of momentarily being forced to think about work.

Presentation 1

General Introduction

Presentation 2

The formation of the limb bud and the identity of the fore and hind limbs

(For background interest this is one of the many papers on the role of Tbx5 in genetic disease. It causes Holt-Oram syndrome: A dominantly inherited malformation syndrome with short stature, upper limb anomaly, minor craniofacial anomalies, and absence of TBX5 mutations: Report of a Thai family )

Presentation 3

Induction of the apical ectodermal ridge and limb outgrowth

Allocation to the AER

AER induction

D/V patterning of the outgrowth

Presentation 4

AER function, the roles of FGF's in limb outgrowth

Presentation 5

Development of the antero- posterior axis


In the interest of actually making research that bit more interesting i have developed "ScopusWhacking". Woo and yay. Aim for the search to return only one research article when provided with just one surname and one other word. Usually works 1/3 of the time. At least it does in bio. God i'm bored…


As promised…

Caption anybody? No prizes well, no prizes that involve the consumption of vinegar shots

M xxx

If Only I Liked Yellow.

Everyone elses is more green...
Propagate this Meme!

October 24, 2005

Development Essay 1 Resources

links to all the research papers

(you may need your athens account details to access them)

Talk 1

Talk 2
Setting up the initial asymmetries in the embryo.

  1. Early patterning of the C. elegans embryo.

  2. Axis determination in _C. elegans: initiating and transducing polarity.

  3. Heads or tails: Cell polarity and axis formation in the early _Caenorhabditis elegans embryo.

  4. Polarization of the C. elegans zygote proceeds via distinct establishment and maintainance phases. (for pictures of localisation)

Talk 3
Skn-1 and EMS development.

  1. Skn-1 a maternally expressed gene required to specify the fate of ventral blastomeres in the early _C. elegans embryos.

  2. Formation of a monomeric DNA binding domain by Skn-1 bZIP and homeodomain elements.

  3. The maternal gene skn-1 encodes a protein that is distributed unequally in early _C. elegans embryos.

Talk 4
Germline development pie-1 and mex-1 genes; transcriptional silencing.

  1. The pie-1 and mex-1 genes and maternal control of blastomere identity in early _C. elegans embryos.

  2. The PIE-1 protein and germline specification in C. elegans embryos.

  3. Repression of gene expression in the embryonic germ lineage of C. elegans.

  4. The C. elegans MEX-1 protein is present in germline blastomeres and is a P granule component. (only need abstract)

  5. Launching the germline in Caenorhabditis elegans: regulation of gene expression in early germ cells.

  6. PIE-1 is a bifunctional protein that regilates maternal and zygotic gene expression in the embryonic germ line of Caenorhabditis elegans.

  7. Germ plasm: protein degradation in the soma.

Talk 5
The C. elegans maternal Notch system.

  1. The maternal genes apx-1 and glp-1 and establishement of dorsal-ventral polarity in the early _C. elegans embryo.

  2. Translational control of maternal glp-1 mRNA establishes asymmetry in the _C. elegans embryo.

  3. Translational control of maternal glp-1 mRNA by POS-1 and its interacting protein SPN-4 in Caenorhabditis elegans.

  4. An inductive interaction in 4-cell stage embryos involves APX-1 expression in the signalling cell

  5. Time dependant responses to glp-1 mediated inductions in early C. elegans embryos.


It's taken me just over 2 (goddamn) hours to find these links.

They make me hurt all over.

I think my eyes may be bleeding
I may never smile again…

oooh a rabbit!!

M xxx

October 07, 2005

Stem cells

Writing about web page

WARNING: this is Biochemistry related. You have permission to run away screaming from your monitor.

ok useful sites i found:

These sites should all refer to stem cell maintainance in vitro. maybe. havn't read them in full yet


A bit on maintaining stem cells.

When used in vitro for research, it is vital that the stem cells used retain their "stemness" or "pluripotency". Without the correct stimulation the cells quicky differentiate into somatic cells, losing their "repopulating capacity"(1). Firstly, the normal procedure for maintaining the culture of cells is as a suspension. The cells must be kept disaggregated; This can be done manually or by nonenzymatic "CDB"(cell dissociation buffer) or enzymatic "CT"(collagenase/trypsin) cell passaging(2). However the CT and CDB methods were found to promote the development of abnormal karyotypes in the cultured stem cells (such as trisomy - particularly of chr17 and 20)

The conclusions were that up to 15 passages with CDB/CT could be used and still mantain a high fidelity to the normal stem cell karyotype(2).

The "cell passaging" seems to refer to the replacement to the medium in which the cells are suspended. Does anyone have a more precise definition?

"hESC"(human embryonic stem cell) cultures are generally grown in conjunction with feeder cells. These cells seem to secrete soluble factors that maintain the stem cells in their undifferentiated state – however the particular factors in question remain unidentified. "mESC"(mouse embryonic stem cells)

1. cDNA cloning of FRIL, a lectin from Dolichos lablab, that preserves hematopoietic progenitors in suspension culture

2. Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers

Maintenance of Pluripotency in Human Embryonic Stem Cells Is STAT3 Independent


these are the speakers for the International symposium on Germ Cells, Epigenetics and Embryonic Stem Cells. I've included mention of where they seem to be focused mostly on one specific area. (The links are just google scholar links to any of their stem cell papers)

Oh and a kitten. But why kittens and stem cells?

That's right.
Because I say so. I love that answer.

*sigh* i know how it feels…

EDIT: Biochemisty is damaging to your mental health. I just found a malformed gummibear in my hairibo and caught myself thinking "I wonder what genetic defect it has? Looks like the morphogen gradient-induced cell differentiation went wrong..." AAaaarrrgggh! development overload. :s

M xxx

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