October 07, 2005

Stem cells

Writing about web page http://forums.warwick.ac.uk/wf/browse/thread.jsp?fid=1299&tid=37226

WARNING: this is Biochemistry related. You have permission to run away screaming from your monitor.

ok useful sites i found:

These sites should all refer to stem cell maintainance in vitro. maybe. havn't read them in full yet

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A bit on maintaining stem cells.

When used in vitro for research, it is vital that the stem cells used retain their "stemness" or "pluripotency". Without the correct stimulation the cells quicky differentiate into somatic cells, losing their "repopulating capacity"(1). Firstly, the normal procedure for maintaining the culture of cells is as a suspension. The cells must be kept disaggregated; This can be done manually or by nonenzymatic "CDB"(cell dissociation buffer) or enzymatic "CT"(collagenase/trypsin) cell passaging(2). However the CT and CDB methods were found to promote the development of abnormal karyotypes in the cultured stem cells (such as trisomy - particularly of chr17 and 20)

The conclusions were that up to 15 passages with CDB/CT could be used and still mantain a high fidelity to the normal stem cell karyotype(2).

The "cell passaging" seems to refer to the replacement to the medium in which the cells are suspended. Does anyone have a more precise definition?

"hESC"(human embryonic stem cell) cultures are generally grown in conjunction with feeder cells. These cells seem to secrete soluble factors that maintain the stem cells in their undifferentiated state – however the particular factors in question remain unidentified. "mESC"(mouse embryonic stem cells)

1. cDNA cloning of FRIL, a lectin from Dolichos lablab, that preserves hematopoietic progenitors in suspension culture

2. Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers

Maintenance of Pluripotency in Human Embryonic Stem Cells Is STAT3 Independent

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these are the speakers for the International symposium on Germ Cells, Epigenetics and Embryonic Stem Cells. I've included mention of where they seem to be focused mostly on one specific area. (The links are just google scholar links to any of their stem cell papers)

Oh and a kitten. But why kittens and stem cells?

That's right.
Because I say so. I love that answer.

*sigh* i know how it feels…

EDIT: Biochemisty is damaging to your mental health. I just found a malformed gummibear in my hairibo and caught myself thinking "I wonder what genetic defect it has? Looks like the morphogen gradient-induced cell differentiation went wrong..." AAaaarrrgggh! development overload. :s

M xxx


- 4 comments by 1 or more people Not publicly viewable

  1. I am running away from my monitor, screaming.

    07 Oct 2005, 08:56

  2. I think your kitten picture is hilarious!

    It's the look of loathing on its face.

    07 Oct 2005, 09:31

  3. Your tag is most accurate. leaves :P

    07 Oct 2005, 10:25

  4. Well, I thought it only fair to warn you all. If only I could run away screaming too. No wait, I do. Regularly.

    Aaaaarrrrgggghhhhhh.................

    07 Oct 2005, 16:37


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