Synthetic Biology talks II

Follow-up to Synthetic Biology from Schreibfaul in Warwick

Title: "Programming bacteria: wiring synthetic sensors and circuits to heterologous outputs"

talk by Christopher Voigt

Three systems in E.Coli:

1. Light Sensor Nature 2005
2. Heat Sensor
3. Logic Gate. in progress. previous Leiber Science2002

Programming Bacteria:

• Competition 6 month time limit. iGEM Genetic Engineering Competition
• a national student competition

One: Light Sensor:

• Bacterial Photography. Change gene expression on light.
• on known photoreceptors in E.coli. just in other organisms.
• Phytochromes in Cyanobacteria, Arabidopsis.
• kinase that is activated on light induction
• compine e.coli osmotic shock sensor with cph1, ouput lacZ

Two: Bacterial Thermometer:

• Heat shock and Cold shock repsonse promoters
• Cold: Hypb, ansb, cspa_x promoter
• Heat: htpG

Synthetic Logic (AND gate):

• Examples of other gates in ecoli, yeast and mammal cells

: 26 Jun 2006 18:26 |  Comments (0) |  Trackbacks (1) |  Report a problem