All 19 entries tagged Agaricus Bisporus

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May 20, 2010

Public Release of Agaricus bisporus var. burnettii JB137–S8 Genome Sequence

The JGI have announced the public release of Agaricus bisporus var. burnettii JB137-S8 genome assembly and annotation, which can be accessed at the following URL:  http://jgi.doe.gov/Agabi_varbur_1

Annotation of the Agaricus bisporus var burnettii (v. 052010) assembly was produced through the JGI annotation pipeline, using a variety of homlogy-based and ab initio gene predictors. ESTs from Agaricus bisporus var bisporus were used. After filtering for EST support, completeness and homology support, a total of 11,289 genes were structurally and functionally annotated.

Statistics on the assembly and annotation of JB137-S8 in comparison to the Agaricus bisporus var bisporus H97 genome are shown in the JGI tables below. Further details can be found on the Info page: 
http://genome.jgi-psf.org/Agabi_varbur_1/Agabi_varbur_1.info.html
AssemblyStats_20100520 AnnotationStats_20100520
Community annotation and analysis of the Agaricus bisporus H97 and JB137-S8 genomes are continuing. Additional contributions are invariably welcomed, please contact MC if you would like to join the community effort.

Please respect the community rules and JGI data release policy for publication rights of the genome-wide analyses:
http://genome.jgi-psf.org/Agabi_varbur_1/Agabi_varbur_1.download.html


May 06, 2010

'MycoCosm' portal for JGI Fungal Genomes and Exploration of Fungal Diversity

Writing about web page http://genome.jgi-psf.org/pages/fungi/home.jsf

MycoCosm

Genome sequences for Agaricus bisporus var bisporus (H97) and var burnettii (JB137-S8) are available through the JGI's 'MycoCosm' portal. 

Fungal Genome sequences and other opportunities to explore Fungal Diversity are layered over a Fungal Tree of Life GUI. This resource should rapidly expand as fungal genomes continue to pour out of the JGI sequencing pipeline.

It's never been easier to explore fungal genomes: Go on, click on a branch!

jgi_logo

JGI_FGP_banner

May 04, 2010

Pre–release of Agaricus bisporus var. burnettii JB137–S8 genome

A pre-release of Agaricus bisporus var. burnettii JB137-S8 genome assembly and annotation is available to the community through the JGI portal.

This pre-release is currently restricted access and a JGI login is required. If you want to access the JB137-S8 portal contact MC for further instructions. It is expected that the Agaricus bisporus var. burnettii JB137-S8 genome
portal will available to the public after 14 May 2010.

Statistics on the assembly and annotation of JB137-S8 in comparison to the Agaricus bisporus var bisporus H97 genome are shown in the JGI tables below:

assemblystats_20100504.jpg

annotationstats_20100504.jpg


Public Release of Agaricus bisporus var bisporus H97 Genome Sequence

H97_HomePage

The Agaricus bisporus var bisporus H97 genome is now available to the public via the JGI portal. Annotations of the v2.0 assembly are available at http://genome.jgi-psf.org/Agabi_varbisH97_2

Detailed statistics on the assembly and annotation of the A. bisporus var bisporus H97 genome can be found on the Info page of the JGI portal: http://genome.jgi-psf.org/Agabi_varbisH97_2/Agabi_varbisH97_2.info.html

H97_browser

Community annotation and analysis of the Agaricus bisporus genomes are continuing. Additional contributions are invariably welcomed, please contact MC if you would like to join the community effort.


Please respect the community rules and JGI data release policy for publication rights of the genome-wide analyses:
http://genome.jgi-psf.org/Agabi_varbisH97_2/Agabi_varbisH97_2.download.html

January 18, 2010

Agaricus bisporus var. burnettii JB137–S8 draft genome

gp_dotplot_2c.jpg The JGI have released a draft genome sequence for a second Agaricus homokaryon JB137-S8 (the tetrasporic Agaricus bisporus var. burnettii).
JB137-S8 genome was sequenced using 454/Illumina technology and assembled by comparisons with the lead H97 genome (Agaricus bisporus var. bisporus).

A brief summary of the JB137-S8 assembly and a dotplot comparison between the var. bisporus and var. burnettii genomes are attached.
jgi_logo JGI are currently progressing annotation of the JB137-S8 genome, after which the standard browser and tools for comparative analysis will become available. Collaborators interested in accessing JB137-S8 data prior to that time should contact MC for further information.
Agaricus bisporus var. burnettii JB137-S8

Main genome scaffold total:                    
2016
Main genome contig total:                      
2527
Main genome scaffold sequence total:           
32.6 MB
Main genome contig sequence total:             
31.2 MB
Main genome scaffold N/L50:                   
8/1.23 MB
Main genome contig N/L50:                      
39/0.163 MB
Number of scaffolds > 50 KB:                  
52
% main genome in scaffolds > 50 KB:            
87.9%

November 05, 2009

Revised Agaricus bisporus var. bisporus (H97) v2.0 genome assembly

The JGI have announced the pre-release of an improved Agaricus bisporus var. bisporus (H97) 8.50x genome assembly, which integrates linkage mapping data.

We are grateful to Anton Sonnenberg, Marie Foulongne and Rick Kerrigan for sharing data and their work mapping various markers to the v2 scaffolds.

This H97 v2.0 genome browser is now available for community annotation inputs.

h97_assembly_nov2009.jpg h97_annotation_nov2009.jpg

In order to access the browser and make annotations, users will need to register with the JGI and the project team will need to approve the request. If you are interested in joining the annotation effort please contact the Agaricus genome team.


October 02, 2009

Pre–release of Agaricus bisporus var. bisporus (H97) v1.0 genome assembly

gbrowser_h97_20091002.jpg

The JGI have announced the pre-release (or ‘internal’ release) of the Agaricus bisporus var. bisporus (H97) 8.50x  genome assembly following automated annotation.

The H97 v1.0 genome browser is now available for community annotation inputs.

In order to access the browser and make annotations, users will need to register with the JGI and the project team will need to approve the request.
If you are interested in joining the annotation effort please contact the Agaricus genome team.

Summary of A. bisporus var. bisporus H97 v1 assembly:
Genome Size: 
30.3 Mbp
Total number of Scaffolds: 27
Total number of EST clusters:           
79,271
Total number of trimmed ESTs:
1,140,141
JGI predicted 10,387 genes with the following average properties:
Gene length (bp):                       
1771.79
Transcript length (bp):
1413.32
Protein length (aa): 426.20
Exons per gene: 6.10
Exon length (bp):
232.25
Intron length (bp): 
71.72
Genes were predicted by the following methods:
EST-derived:                          
3070 (29.6%)
Homology-based:                   
4242 (40.8%)
ab initio:                              
3075 (29.6%)
Genes were validated by the following evidence:
nr protein alignments:               
8638 (83%)
Pfam alignments: 
5357 (52%)
Completeness (5'M-3'*): 9376 (90%)

                 

H97 JGI Project status http://bit.ly/9h4nS | Community annotation plans agaricusgenomeseqactivities_8

August 10, 2009

Agaricus bisporus 8x Assembly Revised

jgi_20090810.jpgThe JGI have issued a revision of the 8X genome assembly for Agaricus bisporus (H97, lead homokaryon).  Jeremy Schmutz of the JGI has provided an assembly report/update JGIupdate_200908

This is an update to the initial draft release (2009 03) and incorporates two additional breaks based on the linkage mapping data provided by Anton Sonnenberg and colleagues. Many thanks to Anton and Jeremy Schmutz for their work on this. 

This draft genome will now go into genome improvement, additional joins may yet be made to complete the chromosomes.


JGI project summaries are available at  http://snipurl.com/ex7l2


Agaricus bisporus 8x Revised Assembly [project ID 4084801 / August 2009]

July 21, 2009

iNquiry Blast server available for Agaricus 8x

An iNquiry Bioinformatics server has been set up at Warwick to facilitate (limited) Blast based* searches of the Agaricus bisporus genome (nt database). This facility should help the community in preliminary screening of the current 8x genome scaffolds prior to the JGI forthcoming sequence release, and permit identification of interesting sequences in advance of their annotations using the JGI genome portal.

*BLASTALL  [ blastn \ tblastn \ tblastx \ psitblastn ]

inquiry_001.jpg inquiry_002.jpg inquiry_004.jpg

Access can be granted to any participants in the genome annotation and interested parties should contact Mike Challen for further information


June 15, 2009

Annotation of Agaricus bisporus genome: community contributions

JGI_20090615Sequence of the Agaricus bisporus H97 haploid genome (ca. 8.5 x coverage) is expected to enter a period of ‘hand-on’ community annotation within the next couple of months.
Anybody interested in participating in the annotation effort should read our community circular genomecircular_20090615.pdf and contact MC for further information





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